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Short protocols in molecular biology Vol.2 : a compendium of methods from Current protocols in molecular biology / editorial board, Frederick M. Ausubel ... [et al.].

Contributor(s): Ausubel, Frederick M | Brent, Roger | Kingston, Robert E | Moore, David D | Seidman, J.G | Smith, John A | Struhl, KevinMaterial type: TextTextPublisher: New York : Wiley, c2002Edition: 5th edDescription: xxxviii, 764 p., ill. ; 28 cmISBN: 0471250929 (cloth); 9780471250920 (cloth)Uniform titles: Current protocols in molecular biology. Subject(s): Molecular biology -- Technique | Molecular biology -- Laboratory manuals | Molecular Biology -- methods -- Laboratory Manuals | Biologie mol�eculaire -- Technique | Biologie mol�eculaire -- Manuels de laboratoire | Biologia molecular | Molecular biology -- Laboratory manuals | Molecular biology | Molecular biology -- TechniqueGenre/Form: Handbooks, manuals, etc.Additional physical formats: Online version:: Short protocols in molecular biology.DDC classification: 581.8 LOC classification: QH506 | .S54 2002Online resources: Publisher description | Table of contents
Contents:
v. 1. Escherichia coli, plasmids, and bacteriophages ; Preparation and analysis of DNA ; Enzymatic manipulation of DNA and RNA ; Preparation and analysis of RNA ; Construction of recombinant DNA libraries ; Screening of recombinant DNA libraries ; DNA sequencing ; Mutagenesis of cloned DNA ; Introduction of DNA into mammalian cells ; Analysis of proteins ; Immunology ; DNA-protein interactions -- v. 2. Saccharomyces cerevisiae ; In situ hybridization and immunohistochemistry ; The polymerase chain reaction ; Protein expression ; Analysis of protein phosphorylation ; Informatics for molecular biologists ; Analysis of protein interactions ; Chromatin assembly and analysis ; Nucleic acid arrays ; Generation and use of combinatorial libraries ; Discovery and analysis of differentially expressed genes in single cells and cell populations.
Summary: Recently expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level Expanded to 2 volumes.
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Includes bibliographical references.

v. 1. Escherichia coli, plasmids, and bacteriophages ; Preparation and analysis of DNA ; Enzymatic manipulation of DNA and RNA ; Preparation and analysis of RNA ; Construction of recombinant DNA libraries ; Screening of recombinant DNA libraries ; DNA sequencing ; Mutagenesis of cloned DNA ; Introduction of DNA into mammalian cells ; Analysis of proteins ; Immunology ; DNA-protein interactions -- v. 2. Saccharomyces cerevisiae ; In situ hybridization and immunohistochemistry ; The polymerase chain reaction ; Protein expression ; Analysis of protein phosphorylation ; Informatics for molecular biologists ; Analysis of protein interactions ; Chromatin assembly and analysis ; Nucleic acid arrays ; Generation and use of combinatorial libraries ; Discovery and analysis of differentially expressed genes in single cells and cell populations.

Recently expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level Expanded to 2 volumes.

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