University of Agricultural Sciences, Bangalore

Includes bibliographical references and index.

Preface Overview Abbreviations and Acronyms 1. Transcriptional Regulation and Chromatin Structure and Function in Eukaryotic Cells 2. Initial Strategic Issues 3. Transcription Initiation Site Mapping 4. Functional Assays for Promoter Analysis 5. Identification and Analysis of Distant Control Regions 6. Identifying cis-Acting DNA Elements within a Control Region 7. Identification of DNA-Binding Proteins and Their Genes 8. Confirming the Functional Importance of a Protein-DNA Interaction 9. Analysis of Transcription Factor-Chromatin Interactions on Endogenous Control Region 10. Identifying and Characterizing Domains of Transcription Regulatory Factors 11. DNA Binding by Regulatory Transcription Factors 12. Transcription and Preinitiation Complex Assembly In Vitro 13. Studying Chromatin Dynamics In Vitro: Chromatin Assembly, Remodeling, and Transcription Index Appendix: Cautions.

"Strategies for studying gene regulation mechanisms have changed dramatically over the past several years. Complete genome sequences for many organisms are now known, and new or improved technologies for chromatin immunoprecipitation, RNA interference, microarrays, and proteomics have become available." "The second edition of this hook has a third author, Craig Peterson from the University of Massachusetts Medical School. In addition to a new chapter on the in vitro analysis of chromatin templates for DNA-binding studies and transcription, this edition has been extensively rewritten and updated to address advances in the field. The second edition retains the approach of the first, covering both the concepts and practicalities of studying the biochemistry of a new transcription factor and how a newly isolated gene is regulated." "Transcriptional Regulation in Eukaryotes is a powerful unique book suitable for advanced instruction in molecular biology. It is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states, providing underlying theory and perspective for the newcomer and the latest techniques for the more experienced."--Jacket.