University of Agricultural Sciences, Bangalore

E-bok.

Introductory Notes -- Record Keeping and Safety Rules -- Exercise 1. Aseptic Technique and Establishing Pure Cultures: The Streak Plate and Culture Transfer -- Exercise 2. Preparation of Culture Media -- Exercise 3. The Growth Curve -- Exercise 4. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep -- Exercise 5. Purification, Concentration, and Quantitation of DNA -- Exercise 6. Large-Scale Isolation of Plasmid DNA by Column Chromatography -- Exercise 7. Amplification of a lacZ Gene Fragment by the Polymerase Chain Reaction -- Exercise 8. Restriction Digestion and Agarose Gel Electrophoresis -- Exercise 9. Southern Transfer -- Exercise 10. Preparation, Purification, and Hybridization of Probe -- Exercise 11. Transformation of Saccharomyces Cerevisiae -- Exercise 12. Isolation of Plasmid from Yeast and Escherichia coli Transformation -- Exercise 13. Protein Assays -- Exercise 14. Qualitative Assay for [beta]-Galactosidase in Yeast Colonies -- Exercise 15. Determination of [beta]-Galactosidase in Permeabilized Yeast Cells -- Exercise 16. Assay of [beta]-Galactosidase in Cell Extracts -- Exercise 17. [beta]-Galactosidase Purification -- Exercise 18. Western Blot: Probe of Protein Blot with Antibody to [beta]-Galactosidase -- Exercise 1A. Isolation and Characterization of Auxotrophic Yeast Mutants -- Exercise 2A. Measurement of pH -- Exercise 3A. Use of the Spectrophotometer -- Exercise 6A. Isolation of Plasmid DNA -- Exercise 10A. Colony Hybridization.

The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment or instruction of introductory courses in the biological sciences.