University of Agricultural Sciences, Bangalore

This is era of plant genomics. Molecular Biology and Biotechnology have taken greatleaps in diverse areas. The complete sequencing of various plants like Arabidopsis, rice, maize and recently wheat has been made available on the public domain. Lots of new genes have been identified, cloned and characterized from different crops. Breeders are using these resources for developing crops with improved productivity, quality and tolerance to various biotic and abiotic stresses. Nutritional enhancement in cereals and legumes are also major targeted areas of research work.

Includes bibliographical references and index.

Protein Assay Chemistry -- Protein Assay Data Analysis -- Biuret Assay -- Lowry Method for Protein Quantitation -- Bradford Method for Protein Quantitation -- Bicinchoninic Acid (BCA) Protein Assay -- Non-Interfering Protein Assay -- Native Polyacrylamide Gel Electrophoresis (PAGE) -- SDS-Polyacrylamide Gel Electrophoresis -- Isoelectric Focusing -- Two Dimensional Gel Electrophoresis -- Maldi-TOF/TOF MS -- Isolation of Genomic DNA from Plants (CTAB Method) -- Miniprep Protocol for Isolation of DNA from Plants -- RNA Isolation and Its Analysis (TriZol Method) -- Restriction Digest of DNA -- Blotting Methods -- Southern Blotting -- Northern Blotting -- Western Blotting -- Polymerase Chain Reaction (PCR) -- Reverse Transcription Polymerase Chain Reaction (RT-PCR) -- Elution of DNA Fragments from Agarose Gel -- Cloning of Gene -- Plasmid Isolation (Alkaline Lysis Method) -- Plasmid Isolation by using Column -- In-Silico Characterization of Gene/Protein -- Expression Analysis of Gene by Quantitative Real-Time PCR -- Key Concepts