University of Agricultural Sciences, Bangalore

Includes bibliographical references and index.

I. Fundamentals of molecular biotechnology. The molecular biotechnology revolution -- Recombinant DNA technology -- Molecular research procedures -- Manipulation of gene expression in prokaryotes -- Heterologous protein production in eukaryotic cells -- Directed mutagenesis and protein engineering -- II. Microbial systems. Microbial synthesis of commercial products -- Molecular diagnostics -- Vaccines and therapeutic agents -- Bioremediation and biomass utilization -- Plant growth-promoting bacteria -- Microbial insecticides -- Large-scale production of proteins from recombinant microorganisms -- III. Eukaryotic systems. Genetic engineering of plants -- Development and use of transgenic animals -- Isolation of human genes -- Human somatic cell gene therapy -- IV. Regulating and patenting molecular biotechnology. Regulating the use of biotechnology -- Patenting biotechnology inventions.

Covers both the scientific principles and industrial applications of recombinant DNA technology. This book explains the principles of this technology, as well as other ancillary techniques such as DNA sequencing, the polymerase chain reaction, and the production of monoclonal antibodies.