TY - BOOK AU - Davis,Leonard G. AU - Dibner,Mark D. AU - Battey,James F. TI - Basic methods in molecular biology SN - 0444010823 AV - QH506 .D39 1986 U1 - 574.88028 19 PY - 1986/// CY - New York PB - Elsevier KW - Molecular biology KW - Methodology KW - Molecular Biology KW - methods KW - Biologie moleculaire KW - Methodologie KW - fast KW - Methode KW - gnd KW - Molekularbiologie KW - Moleculaire biologie KW - gtt KW - Laboratoriumonderzoek KW - Laboratory techniques N1 - Includes bibliographical references and index; 1. The basics of molecular biology -- 2. The tools of the molecular biologist -- 3. General preparations, procedures, and considerations for use of manual -- 4. Cloning vectors and bacterial cells -- 5. Preparation for DNA from eukaryotic cells -- 6. Probing nucleic acids with labeled synthetic probes -- 7. Probing nucleic acids with plasmid-derived probes -- 8. Plasmid DNA preparation -- 9. DNA restriction fragment preparation -- 10. Purification of DNA -- 11. Preparation and analysis of RNA from eukaryotic cells -- 12. Preparation of DNA from bacteriophage clones -- 13. Cloning DNA from the eukaryotic genome -- 14. cDNA cloning into [lambda]gt10 and [lambda]gt11 -- 15. Subcloning into plasmids -- 16. M13 cloning and sequencing -- 17. Further characterization of cloned DNA -- 18. Transfection of mammalian cells in culture -- 19. Protein methods -- 20. General methods -- 21. Specialized methods ER -