University of Agricultural Sciences, Bangalore

This book deals with different basic as well as advanced techniques used in animal biotechnology for molecular characterization of various species/strains/breeds etc., development of genetic markers, various forensic implications, molecular diagnosis of diseases, production of recombinant proteins with respect to vaccinology, detection of mutation (Single Nucleotide Polymorphism / Multinucleotide Polymorphism), cytogenetic and immunogenetic profiles of individuals etc. The book covers molecular biological techniques such as isolation of DNA from tissue/blood/sperm of different organisms, isolation of RNA, qualification, purification of DNA and RNA, detection through electrophoresis (AGE and PAGE), molecular diagnosis by polymerase chain reaction (PCR), molecular characterization by RAPD, AFLP, RFLP and micro satellite, detection of SNPs/MNPs by SSCP and DGGE, forensic uses by DNA finger printing, production of recombinant protein/peptide by cloning and in vitro expression of specific genes; different immunogenetic and cytogenetic techniques for preparation of immuno and cyto-genetic profiles of individuals. The contents of the book have been developed keeping in mind the needs of the undergraduate students, post graduate students, teachers, scientists, laboratory technologists working in the filed of Biotechnology., Molecular Genetics, Molecular Biology, Molecular Biochemistry, Microbiology, Parasitology, Cytogenetics, Immunogenetics, Zoology and other related fields of Veterinary, AGriculture, Fishery, Medical and General stream courses.

Includes bibliographical references [pg. 200.201].