University of Agricultural Sciences, Bangalore

Biotechnology is the controlled engineering of plants suing in vitro methods. As a technology, it is competent to meet the challenge for the increased turnover of biomass for the energy production. The world's demand for food, shelter and wood is expected to double by 2030. Hence, a large scale propagation and rapid multiplication of short duration plants, synthesis of novel, disease resistant, high yielding, disease free and photosyntheticallty efficient plants can no doubt help to solve many of the problems. Tissue culture methods have far-reaching implications for the induction of genetic variability through somatic hybridization, induction of mutants and selection of disease free as well as disease resistant plants. In vitro haploid production will quicken the development of inbreds (instant) and mass multiplication of elite plants. Recent strides in plant molecular biology paved the way of the DNA mediated gene transfer. The structure of blue print of life i.e.DNA, deduced by Watson and Crick (1962) and the action of certain endonucleases in making nick and session of DNA brought out by Arber, Nathan and Smith (1970's) made scientists to transfer genes across taxonomic barriers and irrespective of origin, birth and dignity. From 1980 onwards, large number of transgenic plants were produced both in monocots and dicots by the uptake and incorporation of foreign genome and DNA recombinations. Plant biotechnology is racing ahead in geometric proportion because, unlike biotechnology of animals, plants raise few social, moral and legal issues. Tissue culture and genetic engineering are the most important component of biotechnology. It is envisaged that in future agricultural and forest biotechnology will complement the classical methods breeding and generate novel variabilities. It will open new vistas to enable the improved production of many agronomically important plants. There is a growing need for a simple textbook in in vitro plant breeding. It is out earnest hope that this present book will meet the demand for a comprehensive textbook on tissue culture and genetic engineering for the students of both under and post graduation.