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Principles of gene manipulation / an introduction to genetic engineering / R.W. Old, S.B. Primrose.

By: Old, R. WContributor(s): Primrose, S. BMaterial type: Text

Contents:

Preface -- Abbreviations and conversion scale -- Gene manipulation: an all-embracing technique -- Basic techniques -- Cutting and joining DNA molecules -- Plasmids as cloning vehicles for use in E. coli -- Bacteriophage and cosmid vectors for E. coli -- Cloning strategies, gene libraries and cDNA cloning -- Recombinant selection and screening -- Expression in E. coli of cloned DNA molecules -- Analysing DNA sequences -- The polymerase chain reaction -- Changing genes: site-directed mutagenesis -- Cloning in bacteria other than E. coli -- Cloning in Saccharomyces cerevisiae and other microbial eukaryotes -- Gene transfer to plants -- Introducing genes into animal cells -- Transferring genes into animal oocytes, eggs, embryos and specific animal tissues -- The impact of recombinant DNA technology: the generation of novelty -- Appendix 1: Restriction endonuclease target sites -- Appendix 2: The genetic code and single-letter amino acid designations -- References -- Index.

Action note: Legacy 2018Review: "As in previous editions, the basic philosophy of this extremely popular text remains to present the principles and techniques of gene manipulation in sufficient detail for the non-specialist reader to understand them. However, in the five years since the last edition, molecular biologists have seen a dramatic increase in available techniques and applications. Every chapter in this fifth edition has been extensively revised or rewritten to take these developments into account - including a greatly expanded section on the polymerase chain reaction and its manifold applications; a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics; and extended coverage of gene transfer techniques."--BOOK JACKET. "With entirely new illustrations in two colours throughout, this will remain the textbook of choice for undergraduates and postgraduates alike."--BOOK JACKET.

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Item type	Current location	Call number	Status	Date due	Barcode
Text Book Bank	GKVK Library	575.1 OLD (Browse shelf)	Available		TBB-114595
Text Book Bank	GKVK Library	575.1 OLD (Browse shelf)	Available		TBB-114594

Includes bibliographical references (pages 412-464) and index.

Preface -- Abbreviations and conversion scale -- 1. Gene manipulation: an all-embracing technique -- 2. Basic techniques -- 3. Cutting and joining DNA molecules -- 4. Plasmids as cloning vehicles for use in E. coli -- 5. Bacteriophage and cosmid vectors for E. coli -- 6. Cloning strategies, gene libraries and cDNA cloning -- 7. Recombinant selection and screening -- 8. Expression in E. coli of cloned DNA molecules -- 9. Analysing DNA sequences -- 10. The polymerase chain reaction -- 11. Changing genes: site-directed mutagenesis -- 12. Cloning in bacteria other than E. coli -- 13. Cloning in Saccharomyces cerevisiae and other microbial eukaryotes -- 14. Gene transfer to plants -- 15. Introducing genes into animal cells -- 16. Transferring genes into animal oocytes, eggs, embryos and specific animal tissues -- 17. The impact of recombinant DNA technology: the generation of novelty -- Appendix 1: Restriction endonuclease target sites -- Appendix 2: The genetic code and single-letter amino acid designations -- References -- Index.

"As in previous editions, the basic philosophy of this extremely popular text remains to present the principles and techniques of gene manipulation in sufficient detail for the non-specialist reader to understand them. However, in the five years since the last edition, molecular biologists have seen a dramatic increase in available techniques and applications. Every chapter in this fifth edition has been extensively revised or rewritten to take these developments into account - including a greatly expanded section on the polymerase chain reaction and its manifold applications; a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics; and extended coverage of gene transfer techniques."--BOOK JACKET. "With entirely new illustrations in two colours throughout, this will remain the textbook of choice for undergraduates and postgraduates alike."--BOOK JACKET.

Legacy 2018 UoY

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