University of Agricultural Sciences, Bangalore

Includes bibliographical references and index.

1. Rice Transformation by Agrobacterium Infection / Xiongying Cheng, Ravinder K. Sardana and Illimar Altosaar -- 2. Barley as a Producer of Heterologous Protein / Leena Mannonen, Kristian Aspegren and Anneli Ritala -- 3. Introducing and Expressing Genes in Legumes / Adrian D. Bavage, Mark P. Robbins and Leit Skot -- 4. Use of the GUS Reporter Gene / Joy Wilkinson and Keith Lindsey -- 5. Expression of Recombinant Proteinase Inhibitors in Plants / Dominique Michaud and Thierry C. Vrain -- 6. Detection of Recombinant Viral Coat Protein in Transgenic Plants / Herta Steinkellner and Irina Korschhineck -- 7. Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants / Ravinder K. Sardana, Peter R. Ganz and Anil Dudani -- 8. Tobamovirus Vectors for Expression of Recombinant Genes in Plants / Thomas H. Turpen and Stephen J. Reinl -- 9. Single-Chain Fv Antibodies Expressed in Plants / Udo Conrad, Ulrike Fiedler and Olga Artsaenko -- 10. Characterization and Applications of Plant-Derived Recombinant Antibodies / Rainer Fischer, Jurgen Drossard and Yu-Cai Liao -- 11. Production of Recombinant Antibodies in Plant Suspension Cultures / Jurgen Drossard, Yu-Cal Liao and Rainer Fischer -- 12. Production of Foreign Proteins in Tobacco Cell Suspension Culture / Veronique Gomord, Anne-Catherine Fitchette-Laine and Lise-Anne Denmat -- 13. Transient Gene Expression in Plant Protoplasts / Stefan Schillberg, Sabine Zimmermann and Dirk Prufer -- 14. Stability of Recombinant Proteins in Plants / Dominique Michaud, Thierry C. Vrain and Veronique Gomord -- 15. Trafficking and Stability of Heterologous Proteins in Transgenic Plants / Johnathan A. Napier, Gaelle Richard and Peter R. Shewry -- 16. Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein / Myriam De Neve, Helena Van Houdt and Anne-Marie Bruyns

17. Manipulation of Photosynthetic Metabolism / Martin A.J. Parry, Steven P. Colliver and Pippa J. Madgwick -- 18. Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA / Anne-Marie Bruyns, Myriam De Neve and Geert De Jaeger -- 19. Analysis of N- and O-Glycosylation of Plant Proteins / Anne-Catherine Fitchette-Laine, Lise-Anne Denmat and Patrice Lerouge -- 20. Two-Dimensional Electrophoresis: A Tool for Protein Separation and Processing for Microsequencing / N.P. Eswara Reddy and M. Jacobs.

"In Recombinant Proteins from Plants, leading researchers from around the world present the latest molecular and classical approaches to the separation, analysis, and characterization of recombinant plant proteins. Focusing on the large-scale, cost-effective production of such proteins for use as specialist industrial or therapeutic biomolecules, these authoritative scientists also include promising experimental techniques that will become increasingly important in the future - such techniques as those for the efficient transformation of monocots with Agrobacterium tumifaciens, for optimizing the stability of recombinant proteins, and for exploiting the immunotherapeutic potential of plant-expressed proteins." "Recombinant Proteins from Plants provides a comprehensive and detailed handbook of essential techniques that will benefit both scientists new to the field and established laboratories. It will become the standard reference for even highly skilled researchers working in this most exciting and fast developing field of biology."--Jacket