University of Agricultural Sciences, Bangalore

Includes bibliographical references and index.

1. Detection and diversity of fungi from environmental samples : traditional versus molecular approaches / R. Jeewon and K. D. Hyde -- 2. Functional genomic approaches for mycorrhizal research / A. K. Pandey, H. White and G. K. Podila -- 3. Automated fluoroscence sequencing and troubleshooting / S. Gochhait, D. Malhotra, E. Rai and R. N. K. Bamezai -- 4. mRNA quantitation using real time PCR / S. Gochhait, S. I. Bukhari and R. N. K. Bamezai -- 5. Laboratory practice for the production of polyclonal and monoclonal antibodies / S. Khurana, S. Bhaskar and A. Mukhopadhyay -- 6. Modern techniques for analyzing immunological responses / Satish Khurana, Sangeeta Bhaskar and Asok Mukhopdhyay -- 7. Transcriptome analysis / S. K. Yadav, S. L. Singla-Pareek and A. Pareek -- 8. RNAi technology : a tool for functional validation of novel genes / R. Karan, S. Kumari, S. K. Yadav and A. Pareek -- 9. Molecular matchmaking : techniques for biomolecular interactions / R. Oberoi, P. Kumar and S. K. Lal -- 10. Environmental proteomics : extraction and identification of protein in soil / Z. Solaiman, M. A. Kashem and I. Matsumoto -- 11. DGGE and RISA protocols for microbial community analysis in soil / Z. Solaiman and P. Marschner -- 12. Soil microbial community structureand function assessed by FAME, PLFA and DGGE -- advantages and limitations / P. Marschner

13. Measurement of microbial biomass and activity in soil / Z. Solaiman -- 14. Immuno-technology for the localization of acid phosphatase using native gel bands in Piriformospora indica and other soil microorganisms / R. Malta, U. Pokharel, R. Prasad, R. Oelmuller and A. Varma -- 15. Use of short oligonucleotide primers in random amplified polymorphic DNA techniques for species identification / R. Malla and A. Varma -- 16. Co-cultivation with sebacinales / A. C. Kharkwal, R. Prasad, H. Kharkwal, A. Das, K. Bhatnagar, I. Sherameti, R. Oelmuller and A. Varma -- 17. Quantitative histochemistry : a forgotten tool with new applications / R. Hampp and S. Haag -- 18. Ion cyclotron resonance fourier transform mass spectrometry for non-targeted metabolomics of molecular interactions in the rhizosphere / P. Schmitt-Kopplin, N. Hertkorn, M. Frommberger, M. Lucio, M. Englmann, A. Fekete and I. Gebefugi -- 19. Application of terminal-restriction fragment length polymorphism for molecular analysis of soil bacterial communities / A. Mengoni, E. Giuntini and M. Bazzicalupo -- 20. Molecular symbiotic analysis between Arabiopsis thaliana and Piriformospora indica / B. Shahollari, K. Bhatnagar, I. Sherameti, A. Norma and R. Oelmuller -- 21. Biophysical phenomics reveals functional building blocks of plants systems biology : a case study for the evaluation of the impact of mycorrhization with Piriformospora indica / R. J. Strasser, M. Tsimilli-Michael, D. Dangre and M. Rai -- 22. Analysis of the plant protective potential of the root endophytic fungus Piriformospora indica in cereals / F. Waller, B. Achatz and K.-H. Kogel -- 23. Members of sebacinales confer resistance against heavy metal stress in plants / N. Hahn, A. Varma, R. Oelmuller and I. Sherameti -- 24. Screening of plant growth-promoting rhizobacteria / C. S. Nautiyal and S. M. DasGupta -- 25. Research methods in arbuscular mycorrhizal fungi / A. Gaur and A. Varma -- 26. Field trials of bioinoculants / I. Ortas and A. Varma.

This book presents a wide range of biotechnological methods for application in soil microbiology analysis. These include all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures have been selected with the aim of offering practical guides for immediate use in the laboratory. The systems investigated range from individual molecules and cells to entire eukaryotic organisms, with a focus on bacteria, fungi, mycorrhiza, and higher plants.