University of Agricultural Sciences, Bangalore

Includes bibliographical references and index.

Ribozyme- and sirna-mediated mrna degradation: A general introduction / M. Sioud -- Combination of chemical and enzymatic rna synthesis / R.K. Gaur, A. Hanne and G. Krupp -- Determination of kinetic parameters for hammerhead and hairpin ribozymes / M.J. Fedor -- Real-time monitoring of rna and DNA reactions by fluorescence detection / K.K. Singh, A. Hanne and G. Krupp -- Quantification of ribozyme target rna using real-time pcr / D. Klein, C. Ricordi and R.L. Pastori -- Analysis of ribozyme structure and function by nucleotide analog interference mapping / S. Basu, C. Pazsint and G. Chowdhury -- Analysis of global conformational transitions in ribozymes / D.M. Lilley -- In vivo detection of ribozyme cleavage products and rna structure by use of terminal transferase-dependent pcr / H.H. Chen [and others] -- Identification of efficient cleavage sites in long-target rnas / W.H. Pan and G.A. Clawson -- Selection in vitro of allosteric ribozymes / A. Roth and R.R. Breaker -- Regulation of ribozyme cleavage activity by oligonucleotides / Y. Komatsu and E. Ohtsuka -- Tetracycline-regulated expression of hammerhead ribozymes in vivo / E.T. Bowden and A.T. Riegel -- Ribozyme expression systems / M. Sano and K. Taira -- Design and expression of chimeric u1/ribozyme transgenes / R. Abounader [and others] -- Design and validation of therapeutic hammerhead ribozymes for autosomal dominant diseases / J.J. Fritz [and others] -- Helicase-attached novel hybrid ribozymes / H. Kawasaki [and others] -- Functional gene discovery using hybrid ribozyme libraries / Y. Kato [and others] -- Maxizyme technology / M. Iyo, H. Kawasaki and K. Taira -- Target-site selection for the 10-23 dnazyme / M.J. Cairns and L.Q. Sun -- In vitro selected rna-cleaving DNA enzymes from combinatorial libraries / S. Chakraborti, B. Sriram and A.C. Banerjea -- Nucleic acid sequence analysis using dnazymes / M.J. Cairns and L.Q. Sun -- Crystallization of the hairpin ribozyme: Illustrative protocols / P.B. Rupert and A.R. Ferre-D'Amare -- An experimental method for selecting effective target sites and designing hairpin ribozymes / A. Barroso-delJesus [and others] -- Design and optimization of sequence-specific hairpin ribozymes / C. Romero-Lopez [and others] -- Design, targeting, and initial screening of strsv-derived hairpin ribozymes for optimum helix 1 length and catalytic efficiency in vitro / M.W. Richardson [and others] -- Optimization and application of the group i ribozyme trans-splicing reaction / C. Einvik [and others] -- Repair of myotonic dystrophy protein kinase (dmpk) transcripts by trans-splicing ribozymes / L.A. Phylactou -- General design and construction of rnase p ribozymes for gene-targeting applications / H. Zou [and others] -- In vitro selection of rnase p ribozymes that efficiently cleave a target mrna / K. Kim and F. Liu -- In vitro selection of external guide sequences for directing human rnase p to cleave a target mrna / S. Raj and F. Liu -- Rnase p-mediated inhibition of viral growth by exogenous administration of short oligonucleotide external guide sequence / W. Dunn and F. Liu -- Rnase p ribozyme as an antiviral agent against human cytomegalovirus / P. Trang and F. Liu -- Inhibition of gene expression by nucleic acid enzymes in rodent models of human disease / P.O. Iversen and M. Sioud -- Potential design rules and enzymatic synthesis of sirnas / M. Sioud and M. Leirdal -- Rna interference (rnai) with rnase iii-prepared sirnas / D. Yang, A. Goga and J.M. Bishop -- Rnai expression vectors in mammalian cells / M. Miyagishi and K. Taira -- Gene-array analysis of glioma cells after treatment with an anti-pkcalpha sirna: A general protocol / M. Leirdal and M. Sioud -- Rnai in living mice / H. Hasuwa and M. Okabe -- Construction and transfection of pcr products expressing sirnas or shrnas in mammalian cells / D. Castanotto and J.J. Rossi -- Systemic delivery of synthetic sirnas / M. Sioud and D.R. Sorensen -- Adenovirus-delivered sirna / C. Shen and S.N. Reske -- Rnai expression vectors in plant cells / H. Akashi, M. Miyagishi and K. Taira -- Delivery agents for oligonucleotides / O. Seksek and J. Bolard -- Selection of peptides for specific delivery of oligonucleotides into cancer cells / M. Shadidi and M. Sioud -- Clinical gene therapy research utilizing ribozymes: Application to the treatment of hiv/aids / F.K. Ngok [and others] -- Critical steps in the implementation of hematopoietic progenitor-cell gene therapy using ribozyme vectors: A laboratory protocol / M.P. Boyd [and others].

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