University of Agricultural Sciences, Bangalore

Includes bibliographical references and index.

Introduction to plant virology / Bradley I. Hillman -- Introduction to classical crossprotection / Ron S. Fraser -- History of coat protein-mediated protection / Eric D. Miller, Cynthia Hemenway -- Geminivirus isolation and DNA extraction / Kenneth E. Palmer, Wendelin H. Schnippenkoetter, Edward P. Rybicki -- Caulimovirus isolation and DNA extraction / Simon N. Covey, Rob J. Noad, Nadia S. Al-Kaff, David S. Turner -- Reovirus isolation and RNA extraction / Ichiro Uyeda, Bong-Choon Lee, Yuko Ando, Haruhisa Suga, Yun-Kun He, Masamichi Isogai -- Procedures for plant rhabdovirus purification, polyribosome isolation, and replicase extraction / Andrew O. Jackson, John D. Wagner -- Hordeivirus isolation and RNA extraction / Diane M. Lawrence, Andrew O. Jackson -- Furovirus isolation and RNA extraction / Salah E. Bouzoubaa -- Tobravirus isolation and RNA extraction / Sally C. Taylor -- Tobamovirus isolation and RNA extraction / Sean N. Chapman -- Potexvirus isolation and RNA extraction / Mounir G. AbouHaidar, Huimin Xu, Kathleen L. Hefferon -- Carlavirus isolation and RNA extraction / Gary D. Foster -- Potyvirus isolation and RNA extraction / Philip H. Berger, Patrick J. Shiel -- Trichovirus isolation and RNA extraction / Sylvie German-Retana, Thierry Candresse, Jean Dunez -- Ilarvirus isolation and RNA extraction / Deyin Guo, Edgar Maiss, G�unter Adam -- Bromovirus isolation and RNA extraction / Jozef Julian Bujarski -- Cucumovirus isolation and RNA extraction / Marilyn J. Roossinck, P. Scott White -- Nepovirus isolation and RNA extraction / K. Rogers Wood -- Comovirus isolation and RNA extraction / Joan Wellink -- Carmovirus isolation and RNA extraction / Juana D�iez, Jose F. Marcos, Vicente Pall�as -- Tymovirus isolation and genomic RNA extraction / Adrian Gibbs, Anne M. Mackenzie -- Tombusvirus isolation and RNA extraction / J�ozsef Burgy�an, Marcello Russo -- Luteovirus isolation and RNA extraction / Keith L. Perry, Jane Miller, Richard M. Lister, Mike A. Mayo -- RNA analysis: size and 3' end group determination / Michael Shanks, George Lomonossoff -- RNA fractionation by density gradient centrifugation / Michael Shanks -- cDNA library construction for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- PCR cloning of coat protein genes / Rebecca Stratford -- Antibody production / Carol Brattey, Robert Burns -- Expression library screening / Gary D. Foster -- In vitro transcription and translation / Roisin Turner, Gary D. Foster -- Analysis of coat protein expression cassettes in protoplasts / Jonathan H. Weston, Roisin L. Turner -- DNA sequencing / Jo Badge -- Computer analysis of amino acid sequences : the case of plant virus capsid proteins / Eugene V. Koonin, Arcady R. Mushegian, Valerian V. Dolja -- Preparation of coat protein-containing binary vectors for use in agrobacterium-mediated transformation / Corrin V. Wallis, Margaret I. Boulton -- Potato transformation / Lee Rooke, Keith Lindsey -- Transformation of tomato / Artur J. Pfitzner -- Tobacco transformation / Jennifer F. Topping -- Genetic transformation of wheat / Dong-Fang Chen -- Production of transgenic rice (Oryza sativa subspecies japonica cv. Taipei 309) / Wallace G. Buchholz, Weimin Teng, Delphia Wallace, John R. Ambler, Timothy C. Hall -- Molecular analysis of transgenic rice / Wallace G. Buchholz, James P. Connell, Siva P. Kumpatla, Timothy C. Hall -- PCR analysis of transgenic tobacco plants / Dawn Worrall -- Southern analysis of transgenic tobacco plants / Dawn Worrall -- Detection and quantification of transcript RNA in transgenic plants using digoxigenin-labeled cDNA probes / Kara D. Webster, Hugh Barker -- Western analysis of transgenic plants / Tony J. Lough, Ezequiel Balmori, David L. Beck, Richard L. Forster -- Assaying levels of plant virus by ELISA / Roy Copeland -- Detection of plant RNA viruses by nonisotopic dot-blot hybridization / Vicente Pall�as, Paloma M�as, Jes�us A. S�anchez-Navarro -- Detection and quantification of plant viruses by PCR / Susan Seal, David Coates -- Assaying levels of virus with local lesion hosts / Neil Boonham, K. Rogers Wood -- Field testing resistance of transgenic plants / Wojciech K. Kaniewski, Peter E. Thomas -- Agronomic performance of transgenic plants / Peter E. Thomas, Wojciech K. Kaniewski -- Mechanisms of resistance : expression of coat protein / Ulrich Reimann-Philipp -- Mechanisms of RNA-mediated resistance to plant viruses / Peter Haan -- Detection of risks associated with coat protein transgenics / Roger Hull -- Potential benefits of the transgenic control of plant viruses in the United Kingdom / Ian Barker, Christine M. Henry, Miles R. Thomas, Rebecca Stratford.

Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. The authoritative contributors also discuss the history and mechanisms of coat protein-mediated protection and the key ethical issues surrounding transgenic technology. Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer.