University of Agricultural Sciences, Bangalore

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UNIVERSITY OF AGRICULTURAL SCIENCES

Basic methods in molecular biology / Leonard G. Davis, Mark D. Dibner, James F. Battey.

By: Davis, Leonard GContributor(s): Dibner, Mark D | Battey, James FMaterial type: TextTextPublisher: New York : Elsevier, 1986Description: xi, 388 p. : illustrations ; 26 cmsISBN: 0444010823; 9780444010827Subject(s): Molecular biology -- Methodology | Molecular Biology -- methods | Biologie moleculaire -- Methodologie | Biologie moleculaire -- Methodologie | Molecular biology -- Methodology | Methode | Molekularbiologie | Moleculaire biologie | Laboratoriumonderzoek | Molecular biology Laboratory techniquesAdditional physical formats: Online version:: Basic methods in molecular biology.DDC classification: 574.88028 LOC classification: QH506 | .D39 1986Other classification: 42.13 | WC 4460 | WC 4150 | WD 4150 | BIO 180f | BIO 220f | CHE 810f | CHE 860f
Contents:
1. The basics of molecular biology -- 2. The tools of the molecular biologist -- 3. General preparations, procedures, and considerations for use of manual -- 4. Cloning vectors and bacterial cells -- 5. Preparation for DNA from eukaryotic cells -- 6. Probing nucleic acids with labeled synthetic probes -- 7. Probing nucleic acids with plasmid-derived probes -- 8. Plasmid DNA preparation -- 9. DNA restriction fragment preparation -- 10. Purification of DNA -- 11. Preparation and analysis of RNA from eukaryotic cells -- 12. Preparation of DNA from bacteriophage clones -- 13. Cloning DNA from the eukaryotic genome -- 14. cDNA cloning into [lambda]gt10 and [lambda]gt11 -- 15. Subcloning into plasmids -- 16. M13 cloning and sequencing -- 17. Further characterization of cloned DNA -- 18. Transfection of mammalian cells in culture -- 19. Protein methods -- 20. General methods -- 21. Specialized methods.
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Reference Reference GKVK Library
574.88028 DAV (Browse shelf) Not for loan 99492

Includes bibliographical references and index.

1. The basics of molecular biology -- 2. The tools of the molecular biologist -- 3. General preparations, procedures, and considerations for use of manual -- 4. Cloning vectors and bacterial cells -- 5. Preparation for DNA from eukaryotic cells -- 6. Probing nucleic acids with labeled synthetic probes -- 7. Probing nucleic acids with plasmid-derived probes -- 8. Plasmid DNA preparation -- 9. DNA restriction fragment preparation -- 10. Purification of DNA -- 11. Preparation and analysis of RNA from eukaryotic cells -- 12. Preparation of DNA from bacteriophage clones -- 13. Cloning DNA from the eukaryotic genome -- 14. cDNA cloning into [lambda]gt10 and [lambda]gt11 -- 15. Subcloning into plasmids -- 16. M13 cloning and sequencing -- 17. Further characterization of cloned DNA -- 18. Transfection of mammalian cells in culture -- 19. Protein methods -- 20. General methods -- 21. Specialized methods.

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