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UNIVERSITY OF AGRICULTURAL SCIENCES

Proteins and proteomics : a laboratory manual / Richard J. Simpson.

Contributor(s): Simpson, Richard JMaterial type: TextTextPublisher: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, 2003Description: xiii, 926 pages : illustrations ; 29 cmISBN: 0879695536; 9780879695538; 0879695544; 9780879695545Subject(s): Proteomics -- Laboratory manuals | Proteins -- Laboratory manuals | Proteins | Proteomics | Proteins | Proteomics | Prot�eomique -- Manuels de laboratoire | Prot�eines -- Manuels de laboratoire | Prot�eines | Prot�eomique | protein | Proteins | Proteomics | Labortechnik | Proteine | Proteomanalyse | Reinigung | Eiwitten | Prote�inas | Manuais de laborat�orio | Prot�eomique | Prot�eine | Technique de laboratoireGenre/Form: Laboratory Manual | Laboratory manuals. | Laboratory manuals. | Manuels de laboratoire.Additional physical formats: Online version:: Proteins and proteomics.; Online version:: Proteins and proteomics.DDC classification: 574.19296 LOC classification: QP551 | .P697776 2003Other classification: 35.75 | WC 4170 | WD 5100 | 35.75. Online resources: Companion website of supplemental material
Contents:
Introduction to Proteomics -- One-dimensional Polyacrylamide Gel Electrophoresis -- Preparation of Cellular and Subcellular Extracts -- Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients -- Reversed-phase High-performance Liquid Chromatography -- Amino- and Carboxy-terminal Sequence Analysis -- Peptide Mapping and Sequence Analysis of Gel-resolved Proteins -- Use of Mass Spectrometry in Proteomics -- Proteomic Methods for Phosphorylation Site Mapping -- Characterization of Protein Complexes -- Making Sense of Proteomics: Using Bioinformatics to Discover a Protein's Structure, Functions, and Interactions.
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Item type Current location Call number Status Date due Barcode
ST Book Bank ST Book Bank GKVK Library
574.19296 SIM (Browse shelf) Available STB-632

Includes bibliographical references and index.

1. Introduction to Proteomics -- 2. One-dimensional Polyacrylamide Gel Electrophoresis -- 3. Preparation of Cellular and Subcellular Extracts -- 4. Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients -- 5. Reversed-phase High-performance Liquid Chromatography -- 6. Amino- and Carboxy-terminal Sequence Analysis -- 7. Peptide Mapping and Sequence Analysis of Gel-resolved Proteins -- 8. Use of Mass Spectrometry in Proteomics -- 9. Proteomic Methods for Phosphorylation Site Mapping -- 10. Characterization of Protein Complexes -- 11. Making Sense of Proteomics: Using Bioinformatics to Discover a Protein's Structure, Functions, and Interactions.

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